HAEMATOXYLIN

Colour Index #75290.
C16H14O6. F.W.302.29 CAS#5172-8-2
For use in Mayer's acid, Harris's alum haematoxylin, in Weigert's and Heidenhain's iron and other haematoxylins stains. Used in histology, cytology and also with plastic embedded tissues.
Chen, Chang S., J.Arch. of Path., 93,334 (1972).
Fax for 500g or 1kg price, we are most competetive.

C107
Haematoxylin, certified, C.I. 75290
25g
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C1071
Haematoxylin, certified, C.I. 75290
100g
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C1075
Haematoxylin, certified, C.I. 75290
500g
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HEPES BUFFER

(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
C8H1804N2S F.W. 238.30 CAS #7365-45-9

Assay..................... > 99%
pH Range................ 7.0-8.0
A(260) (1.0M in H20)...... < 0.05
Ash...................... < 0.1%

Good's buffer

C018
Hepes buffer
25g
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C0181
Hepes buffer
100g
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HEXAMETHYLDISILAZANE (HMDS)

CAS# 999-97-3

F.W. 161.40 B.P. 126.7°C. For fast preparation of soft insect tissues, also bacteria and some other "hardy" specimens in lieu of CPD.
Method: After complete dehydration up to 2 changes in abs. ethanol, treat specimens for 10min. in 1:1 ethanol : HMDS and then 3x10 minutes in pure HMDS. Dry specimen on filter paper in a glass Petri dish with lid ajar within a fume hood.
Slower drying by refrigerating the Petri dish overnight (and warm up before opening to avoid condensation) may be beneficial with sensitive specimens.Int.J.Insect.Morph.Embryol., 12(4),201 (1983); Stain Tech., 58(6),347 (1983)

C108
Hexamethyldisilazane
100mL
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HISTOCRYL

For thin 'one micrometre' sections. By London Resin. Specially formulated for light microscopy. A low toxicity acrylic resin, it is easy to prepare and handle and is more adaptable for staining procedures when compared with the epoxy resins.


Benzoyl peroxide MSDS

Components:

  • 500mL Histocryl resin
  • 50g Benzoyl peroxide
  • 10mL Accelerator
C032
Histocryl resin kit
ea
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INDIGO CARMINE

CAS# 860-22-0, Colour Index #73015

Shunway's stain for animal embryos. Used with acid fuchsin for staining Negri bodies. Stain Tech., 5,34 (1928) Counter‐stain to haematoxylin for staining vaginal smears. Am.J.Anat., 61,505 (1937)

C111
Indigo Carmine, C.I. 73015
25g
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C1111
Indigo Carmine, C.I. 73015
100g
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C1115
Indigo Carmine, C.I. 73015
500g
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INDIUM TRICHLORIDE

CAS# 10025-82-8

Anhydrous. Metal stain for nucleic acid. F.W. 221.18. J.Biophys.Biochem.Cytol.,11,257 (1961)

C112
Indium trichloride
5g
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JB-4 EMBEDDING KIT

A water soluble embedding media which is based on Glycol Methacrylate (GMA) plastic embedding. It is intended for use in preparing samples for high resolution microscopes (HRLM). The catalysed monomer acts as a dehydration and infiltration agent; therefore, complete dehydration through 100% ethanol is not necessary (although recommended for large or dense tissues). Conventional paraffin sections have a greater degree of shrinkage and produce inadequate morphology when compared to JB-4. Features:
Thin sections (.5-2.0 micron) with excellent morphological structure preservation. Water-clear blocks; casts in 90 minutes max. at room temperature. Good lipid and enzyme retention when processing at low temperatures (4°C). Removal of JB-4 resin prior to staining is not necessary. Clearing agents such as xylene and chloroform are not needed. Higher clarity and contrast than with paraffin sections. Easy processing of difficult specimens such as calcified bone and delicate embryonic tissue. All components may be stored at room temperature.


References: 1.Hofman E.O. & Flores, T.R., High Resolution LM in Renal Path., Amer. J. of Clin. Path., 76:5 (1981) 2.Beckstead, J.H., Blood, 57(6):1008 (1981) 3.Brinn, N & Pickett, P., J. Histotech., 2(5):125-130 (1979) 4.Block, Matthew H., et al., Lab. Med., 13(5)(1982) 5.Helander, K.G., J. Microscopy, 132:223 (1983) 6.Cole, M.B., J. Microscopy, 127:139 (1982) 7.Higuchi, S., et al., Stain Tech., 54(1):5-12 (1979) 8.Van DeVeldt, S., Am. J. Clin. Path.,73:121 (1980) 9.Horton, W.A., J. Histochem. Cytochem., 31:417 (1983) 10.Tacha, D.E. & Richard, T.C.., J. Histotech., 4(2):59 (1981)


JB-4 kit components:

  • C611 JB-4 solution A only, 750ml MSDS
  • C612 JB-4 solution B only 50ml MSDS
  • C613 JB-4 catalyst 12g MSDS
C610
JB-4 embedding kit
ea
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C611
JB-4 solution A
750mL
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C612
JB-4 solution B
50mL
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C613
JB-4 catalyst
12g
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JB-4 Plus™ EMBEDDING KIT
JB-4 Plus offers all the features as JB-4 with the following additions: It produces a weaker exothermic reaction than JB4 and this benefits temperature sensitive tissues. It also produces harder blocks which is ideal for dense samples, such as bone. Store at RT.
Components:
  • C616 JB-4 Plus solution A, 500mL MSDS
  • C617 JB-4 Plus solution B, 45mL MSDS
  • C618 JB-4 Plus catalyst, 8g MSDS
C615
JB-4 Plus embedding kit
ea
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C616
JB-4 Plus solution A
500mL
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C617
JB-4 Plus solution B
15mL
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C618
JB-4 Plus catalyst
8g
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JENNER'S STAIN

CAS# 62851-42-7

A Romanowsky-type stain for peripheral blood smears.

C117
Jenner's stain, certified
25g
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C1171
Jenner's stain, certified
100g
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C1175
Jenner's stain, certified
500g
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