Reagent, ACS.
Pb(CH₃COO)₂ :3H₂O F.W. 379.33
Specifications: Assay 99.0-103.0%; chloride 5ppm; copper 0.002%; insoluble matter 0.01%; iron 0.001%; nitrate and nitrile (as NO3) to pass test. For the preparation of lead "stains".
Lead is cumulative in the body and is toxic.

Trihydrate
Pb₃(C₆H₅O₇)₂:3H₂O. M.W. 1053.82 CAS#512-26-5
"Stain" for ultra-thin EM sections.
Lead is cumulative in the body and is toxic.

Reagent, ACS
Pb(NO₃)₂ F.W. 331.21 CAS#10099-74-8
Minimum Assay 99.5%. Metal "Stain" for Ultra-thin EM sections.
Sato, T., J.Electron Micro., 16,733, (1967)
Lead is cumulative in the body and is toxic.

CAS No.: [12627-53-1]
Appearance: Dark-green with metallic crystals
Leishman Stain is used generally in haematology and tropical medicine to differentiate and identify leucocytes, malarial parasites and trypanosomes.
References:

1. Leishman, W.B. Br. Med. J. 1901,2, 757-758.
2. Wright, J.H. J. Med. 1902,7, 138-144.

Acid Green 5. For use as a cytological counter‐stain and in Little's modification of the Masson's trichrome stain. Minimum dye content 65%.

Lowicryl resins are highly cross-linked acrylate-based embedding resins, designed for use over a wide range of embedding conditions. These resins provide low viscosity at low temperatures. The K4M kit is usable to -35°C and is a polar (hydrophilic) medium, while the HM20 kit can be used down to -70°C and is nonpolar (hydrophobic). Lowicryl's K11M and HM23 have properties similar to K4M and HM20, but can be used at temperatures 20 degrees lower than others: K11M at -60°C, HM23 at least -80°C. All of these resins are photopolymerised by long wavelength (360nm) ultraviolet light. Since the initiation of the polymerisation is largely independent of temperatures, blocks may be polymerised at the same temperatures used for infiltration. The resin may also be chemically polymerised at 60°C.

Lowicryl MonoStep Single Component Embedding Media
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimises chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling. Store at room temperature.

C440 contains: 3 x 225mL Monomer E, 1 x 130g Crosslinker D, 4.8g Initiator C

C430 Lowicryl K4M MSDS - ask
C435 Lowicryl K4M monostep (polar) MSDS - ask
C460 Lowicryl HM23 MSDS - ask

MSDS

The London Resin Company's acrylic "LR Gold" is the resin of choice when the antigenicity of the tissue is quite fragile. LR Gold is cured by the blue fraction of white light and this is possible at low temperatures. LR Gold is especially designed for low temperature (-25°C) embedding of fresh or lightly aldehyde-fixed tissue. Choice of initiator will determine polymerisation method - UV to intense white light. A PVP concentration above 20% may improve EM morphology but can also result in very high viscosities at low temperatures. LR Gold is non-toxic and has a low viscosity.

For "cold curing" with light of non-osmicated tissue.

Note: Minor acid number variation of some resins results in a colour change when the mixture is made up: from clear to amber to pink. This does not affect resin performance.

A very low viscosity (8cps), non-toxic resin, suitable for both light and electron microscopy. LR White is a polar monomer polyhydroxylated achromatic acrylic resin. Curable by heat or light. Sections of polymerised LR White resin are hydrophilic. This characteristic allows immunocytochemistry reagents and stains to easily penetrate the sections. Etching of sections is not necessary. LR White sections show minimal non-specific staining.
Its low viscosity makes it ideal for infiltrating decalcified bone and teeth as well as plant tissue. It is available in medium and hard grade.
Distance, time and high temperatures during shipping from the manufacturer would age catalysed resin too much, we purchase and ship LR White uncatalysed, but the material is supplied with catalyst (benzoyl peroxide) and detailed instructions, but read in the linked User Notes how to catalyse without ruining the whole bottle. Catalysed LR White keeps refrigerated for over one year and embedded specimens can be cured using heat only. For cold curing with light, accelerator must be added.
Newman, G.R. Use and abuse of LR White. Histochem.J. 19:118 (1987)
Newman, G.R. LR White embedding medium for colloidal gold method. In: Colloidal Gold: Principles, Methods, and Applications, Vol.2 (Hayat, M.A., Ed.). Academic Press, San Diego and London (1989).
Note: Minor acid number variation of some resins results in a colour change when the mixture is made up: from clear to amber to pink. This does not affect resin performance.

Benzoyl peroxide MSDS

C024C is catalysed and needs to be shipped cold from Townsville, preferably no further than Sydney. It is not a DG.

A technique producing an electron dense osmiophilic polymer when lucifer yellow is injected into cells. Lucifer yellow can also be viewed in living cells as a dye marker and is more sensitive than any other dye. Cell Tissue Res., 204,37 (1979). J.Histochem. Cytochem., 30,189 (1982). Science, 216,943 (1982); 214,801 (1981) 225,851 (1984). Cell, 14,741 (1978). Fluorescent dye: Nature 292,17 (1981)

M = 246.48g/mol
AR grade

F.W. 116.08 Crystalline, repurified, A.C.S. Assay 99.5% toxilic acid; cis-butenediodic acid. M.P. 132-134°C.

This stain is sometimes favoured over Jenner's and it too is a combination of Eosin Y and Methylene Blue. Jenner's versions required aging, but this chemically polychromed Romanowsky type stain may be used immediately after preparation.
The Biological Staining Commission does not certify May-Gruenwald. Of course our stain performs as intended. The powder is dark green and soluble in water and methanol.

Otherwise known as basic blue 20. Used as a counter‐stain in Almann's aniline acid fuchsin techniques for mitochondria in Kurnick's Methyl Green‐Pyonin stain for nuclei and cytoplasmic granules of liver cells.
Boyd, T.J.B., and Mitchell, H.R.,
Marker for DNS at acid and natural conditions. Analy.Biochem., 13,28 (1965)

Chemical Formula: C₅H₈O₂

Also called Acid Orange 52.
Used as a stain for pituitary acidophils with the Wilson‐Ezrin method, in the Kreyberg stain and in Flemming's triple stain on plant material.

Indicator Dye
Free acid, high purity, additive to various media for colour indication of pH.

Technical grade, for industrial use.

Used with Gentian Violet and Bismarck Brown Y for staining metachromatic granules of diphtheria organisms. Minimum dye content 75%.

Used to distinguish autonomic nerves.
Richardson K.C., Anat.Red., 164,231 (1969). General stain for animals, histology. Dougherty, M.M., and King, J.S., A simple, rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and methylene blue. Stain Tech., 59,149 (1984). Shroeder H.E., Rossinsky, K., and Muller, W., An established routine method for differential staining of epoxy‐embedded tissue sections. Microsc.Acta 83,111 (1980)

Molecular sieve type 4A desiccant
A desiccant for drying ethanol, acetone and ammonia. In pellets form 2.5x5mm. The pellets may be dried and re‐used like silica gel. Do not place solvent saturated pellets into an oven!
For silica gel see C81 on page C8

For use in supra‐vital staining of living blood cells. Minimum dye content 50%.

Water soluble. For negative staining of bacteria.
With Basic Fuchsin, staining of bacteria spores. Dorner, Le Lait, 6,8 (1926)

Note that Nile Blue A, sulphate and Nile Blue, chloride have the same CI number; they act and stain similarly. Differentiates melanins and lipofuscins; used in fat staining. Lillie, Stain Tech., 31,151 (1956). J.Histochem., 4,377 (1956). Z. Analyt. Chem.,260,290 (1972). For staining bone sections. Cytologie,11,261 (1940)

(Methyl-5-norbornene-2,3-dicarboxylic anhydride)
(The trademark NADIC is owned by another company).
F.W. 178.19, CAS: 25134-21-8. Epoxy hardener. B.P. 135°C

(Nonenyl Succinic Anhydride)
Epoxy hardener. B.P. 126-130°C.