• Histology automated tissue processor

Note: Quorum Technologies have discontinued stand-alone glow discharge units. This feature is now included only in the KSC7620 and the KQ150 series as an optional accessory. We can supply a non-Quorum stand-alone glow discharge system.

The primary application of glow discharge is as a technique for the surface modification, or 'wetting', of newly-evaporated transmission electron microscopy (TEM) carbon support films.

For one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air, the PELCO easiGlow™ uses an automated and quick cycle with fully selectable parameters. The microprocessor controlled system also offers full manual control for all parameters and an advanced protocol programming feature for custom designed glow discharge applications. Parameters and protocols can be stored to facilitate consistent glow discharge results. Open the shipping box, plug it in, and you can start.

The PELCO easiGlow™ Glow Discharge Cleaning System is a compact, quick and easy to use standalone system. It is primarily designed for hydrophilisation of TEM carbon support films, which have the tendency to be hydrophobic. A glow discharge treatment with air will make a carbon film surface negatively charged (hydrophilic) which allows aqueous solution to spread easily.

With increasingly demanding imaging applications, using clean and consistent quality TEM grids and supports has become more important than ever. The PELCO easiGlow™ is an affordable and easy to operate glow discharge system for any TEM lab.

The practical chamber size with its newly designed glow discharge head and adjustable stage with glass slide holder allows for easy loading and fast turnaround times. The system is fully microprocessor controlled and includes an intelligent touch screen device for control and display of operation parameters. The PELCO easiGlow™ supports both hydrophilic and hydrophobic treatment with either negative or positive charge and includes two separately controlled gas inlets. The required vacuum level is set by an electronically controlled precision proportional valve. This novel approach eliminates manual setting with a needle valve. A gentle venting procedure ensures that the TEM grids are not disturbed when the system is vented.

Optional Accessories

For more information and pricing for these accessories ‐ ask
PELCO easiGlow™ and GD7 Vacuum Systems are available in 110V, 60Hz ‐ ask

Lynx II Automated Tissue Processor for Histology and Microscopy is a state-of-the-art tissue processor which is compatible with all plastic resins and paraffin waxes.

Introduction
Lynx II is designed to be the successor of the Lynx Tissue Processor with several enhancements including the optional processing of larger size samples for Histology.

The Lynx II holds 24 reagent vials for EM processing. Optional HP (Histology processing) may be done with 12 larger size reagent vials for HP processing. In both EM and HP modes, Lynx II has two, independently controlled, heating/cooling stations.

Operational Overview of Lynx II
Lynx II is a batch mode, tissue processing system. The tissues may be processed for EM or HP. Tissues of either type are processed in the same manner: Samples are mounted on the sample arm and reagents are loaded on the reagent carousel. Based on the selected program by the user, the Lynx II rotates the reagent carousel until the selected reagent station is at the processing station. At the processing station the sample is lowered in the reagent. Based on the selection of agitation and vacuum, the Lynx II periodically moves the sample arm to provide agitation and applies vacuum when the agitation is not in progress. Once the programmed time in a given reagent is over, the sample is raised and the next reagent is rotated in the processing station.

The operator may select the processing for EM or HP by the program numbers. Programs 1 through 10 are used for EM processing and programs 11-20 are used for HP processing. In addition to the proper program number selection, it is the responsibility of the end user to use the proper carousel positions and the appropriate type of reagent vials based on the selection of EM vs. HP processing. Once the unit is properly configured for EM or HP processing, the operator loads the reagent carousel and the samples, selects the desired program and starts the run.

At the end of the run, the sample is retained in the last reagent until the operator removes the sample.

Features

• 12 reagent vials for HP processing, each 125mL volume
• 24 reagent vials for EM processing, each 25mL volume
• 10 Programs for EM and 10 programs for Histology
• Two independently controlled heating/cooling stations
• Easy replacement of reagent carousel
• Paraffin processing
• Sample agitation
• Vacuum infiltration during processing
• Choice of plastic and metal containers for improved chemical compatibility and heat transfer
• Optional external media based interface to a PC for ease of programming and allowing for the editing of programs on a PC and the transfer of programs between the Lynx II and the PC
• Built-in fume extraction and ability to connect the discharge hose to lab exhaust system
• Internal battery back-up during power fail to protect the samples
• Ability to continue run after power interruption
• User Friendly Operator Interface
• Optional external UPS for continued processing during longer power outages

Functional Specifications
System Configuration
1. Lynx II is designed with the following functional modules.

• Rotary mechanism to rotate the reagents
• EM and HP reagent carousel
• Up/Down mechanism to move the sample in and out of the reagent as well as to provide the agitation
• EM and HP Sample arms
• Tight seal for all reagent containers except for the one at the processing station.
• Vacuum pump and a pressure sensor or a switch
• Two Peltier operated heating/cooling station with independent temperature sensors and controls
• Fume extraction fan and air flow sensor

2. Reagent Carousel
A reagent carousel may be installed, either a 24-position carousel for holding EM reagents or a 12-position carousel for holding HP reagents. The EM carousel holds cylindrical vials that are approx. 1.2" in diameter and 2.5" tall whereas HP vials are approx. 2.2" diameter (to hold 1.75" x 1.2" cassette flat) and 2.5" tall. The carousel is approx. 12" in diameter. Each vial has a flange for ease of handling. The vials are available in either plastic or aluminium. The vials may be mixed and matched between the metal and the plastic vials on a given carousel for certain kinds of processing.

The Lynx II has a built in sensor to detect that the proper reagent vial types (EM vs. HP) are installed on the unit before starting a run. Ability to detect only one of the two vial types is sufficient.

3. Sample Arm
The sample arm holds either an EM sample holder assembly or an HP sample holder assembly. Each sample holder assembly is designed to mount on the arm at a location to centre the samples in its respective reagent container. The Sample Arm top may be changed to accommodate different samples.

4. Agitation and Vacuum
The arm provides a gentle agitation (approx. 5 mm up and down travel at a rate of 2.5 mm per second or one up/down agitation per second) once the sample is in the reagent container. Five agitation strokes are executed in 5 seconds and repeated every 45 seconds. If vacuum infiltration is selected, the agitation will be applied for 5 seconds followed by 40 seconds of vacuum. These agitation-only or the agitation-vacuum cycles will be repeated for the duration of the station time.

The sample holder assembly has a fitting to attach the vacuum hose from the vacuum pump.
5. Heating-Cooling Stations
The Lynx II provides 2 independent, identical heating-cooling stations (HCS). Each HCS is heated or cooled by a Peltier module and has its own temperature sensor. One HCS is located at the sample processing station and the second one is located one position prior to the sample processing station. This arrangement allows for the pre-heating or pre-cooling of the reagent for a program where the reagents are arranged in a sequential, contiguous manner.

Each HCS can be programmed to be between 4 deg. C and 65 deg. C. The accuracy of the actual temperature will be ± 2 deg. C measured on the surface of the Heating – Cooling station. The set point at the HCS surface is reached within 7 minutes of the arrival of the reagent at the station.

6. Fume Control
Lynx II provides a fume extraction fan to direct fumes from the reagent/ sample area to an exhaust port. An external hose may be connected to the unit and the hose may be directed to a fume hood or to laboratory exhaust system. The fume extraction is designed to ensure that the fumes are diverted away from electronics and the mechanism components.

7. Lid/Cover
An acrylic cover with a latch is part of the fume control system. Controller of the system can detect if the cover is latched or not. By default, a run cannot be started (and will pause) if the cover is not in place. However,there is a software override will allow for the running of the unitt without closing the cover.

8. Seal for the Reagent Vials
A tight seal is provided to cover all reagent vials except for the one under the processing station.

Reagents Used
Materials for various components and subsystems of Lynx II are compatible with the following reagents during the processing.

HP Processing

• Formalin
• Alcohol
• Xylene
• Xylene Substitutes
• Water
• Paraffin
• 10% bleach solution (to wipe clean the system)

EM Processing

• Buffers
• Osmium Tetroxide
• Ethyl Alcohol
• Acetone
• Propylene Oxide
• Epoxy/acrylic resins

9. Operation During Power Outage
The Lynx II contains a small internal battery (that is capable of moving the sample from up position to down position and to move the carousel in the next reagent position at least three times without being re-charged) to preserve the sample during a power outage. When the electronics in Lynx II detects that the A/C power is about to fail, the Lynx II will shut down the power to the Peltier elements at both heating/cooling stations. Next, it will ensure that the samples are down in a reagent container. If the samples are out of a reagent vial (since Lynx II was just about to rotate the carousel to the next reagent position or was in the middle of the rotation), the carousel will be rotated by one position and then the samples will be lowered in to the reagent at the processing station. The time when the power fail warning was activated will be recorded and the unit will go in the stand by mode.

Once the power is restored and is maintained for at least 30 seconds, Lynx II will resume the run. As Lynx II resumes run, the station time count down does not resume until the heating/cooling stations have reached the desired temperatures. The station time countdown does not begin for additional 60 minutes if the temperature at the processing station or at the previous station was above 55 deg. C during an HP run. This feature ensures that the paraffin at either of the two stations completely melts before moving to the next station

Specifications:

Lynx II accessories and spare parts
L12610 Histology Kit
(Histo Sample Holder, Histo Sample Arm, Histo Carousel, Power Cord, Operator Manual, Histo Aluminium Containers (24), Histo Plastic Containers (36), Plastic Caps (36), Exhaust Hose, Small Cassettes (100), 2 Sample Record Note Pads, Spare Fuses.

L12612 Electron Microscopy Kit
(EM Sample Holder, EM Sample Arm, EM Carousel, Loading Jig, Power Cord, Operator Manual, Aluminium Containers (24), Plastic Containers (72), Plastic Caps (72), Exhaust Hose, Small Baskets (100), Large Baskets (100), Basket Lids (100), 2 Sample Record Note Pads, Spare Fuses.

L12655 EM Reagent Vials, Bag of 72
L12656 EM Regent Vial Cap, Bag of 72
L12657 HP Reagent Vials, Bag of 36
L12658 HP Reagent Vial Cap, Bag of 36
L12659 EM Aluminium Container (Box of 24)
L12660 HP Aluminium Container (Box of 12)
L13154 EM Sample Holder Assembly (Basket Stem Assembly)
L12663 HP Cassette Holder Assembly
L12661 Vacuum Pump Tubing (1 each)
L12662 Vial Seal Assembly
L12995 EM Sample Arm Seal
L12996 HP Sample Arm Seal
L12952 EM Carousel (Without Aluminium Containers)
L12953 HP Carousel (Without Aluminium Containers)
L12664 Replacement Back-up Battery
L12665 Lynx II Operator Manual
L12667 Lynx II Service Manual

The Lynx II for Microscopy comes with: EM Sample Holder, EM Sample Arm, EM Carousel, Loading Jig, Power Cord, Operator Manual, Aluminium Containers (24), Plastic Containers(72), Plastic Caps (72), Exhaust Hose, Small Baskets (100), Large Baskets (100), Basket Lids (100), 2 Sample Record Note Pads, Spare Fuses.

The Lynx II for Histology comes with: Histo Sample Holder, Histo Sample Arm, Histo Carousel, Power Cord, Operator Manual, Histo Aluminium Containers (24), Histo Plastic Containers(36), Plastic Caps (36), Exhaust Hose, Small Cassettes (100), 2 Sample Record Note Pads, Spare Fuses.

The Lynx II for Microscopy and Histology comes with everything found in both units.

All of the above accessories may be purchased separate or in the kit.

Consumables for Lynx I and II are on page H3

Lynx 1 has not been produced for many years. Parts are supplied from a stock but these will exhaust at some point. Please consider initiating the purchase of Lynx 2. A trade in of AUD500 is given when purchase of a Lynx 2 system.