Lab-Tek® Chamber Slide™, Lab-Tek® II Chamber Slide™ and Lab-Tek® II CC2™ Chamber Slide™ Products
The growth of various cell types on plastic, soda lime glass and borosilicate coverglass were examined. The surfaces were either unmodified, coated with poly-lysine, or stably surface modified with non-biological reagents as described by Kleinfield, et al. (1988). All substrates were assembled into Lab-Tek® and Lab-Tek®II Chamber Slide™ products.
| Product Features | Lab-Tek® | Lab-Tek® II | Lab-Tek® II CC2 |
| Configurations | 1, 2, 4, 8, 16 well, flask style | 1, 2, 4, 8 well | 1, 2, 4, 8 well |
| Media chamber material | Proprietary cell culture grade polystyrene (PS) | Proprietary cell culture grade polystyrene (PS) | Proprietary cell culture grade polystyrene (PS) |
| Slide Materials | Soda lime glass or Permanox® plastic | Soda lime glass | Soda lime glass |
| Cover | Clear polystyrene, no handle, facilitates microscopic viewing | Clear polystyrene, with handle for aseptic manipulations | Clear polystyrene, with handle for aseptic manipulations |
| Sealant | Non-toxic medical grade silicone • Remains on slide after detachment | Biocompatible acrylic adhesive | Biocompatible acrylic adhesive • Remains with media chamber after detachment |
| Reagent ‘isolator’ | Created by removable silicone gasket | Created by hydrophobic border | Created by hydrophobic border |
Chamber separation | No separation tool required | Requires separation tools | Requires separation tools |
| Growth Surface | For adherent cell culture Glass slides • 6-stage high purity water wash Permanox® plastic • Cell culture treated Polystyrene • Cell culture treated | For adherent cell culture RS treated glass slide Proprietary wash | For adherent cell culture chemically modified surface that’s optimal for fastidious cells Mimics a poly-lysine coated. |
| Biological testing | BHK-21, Hep-2 cells | BHK-21, Hep-2 cells | Primary chick brain neurons, Hep-2 cells |
| Leak tested | Yes | Yes | Yes |
| Slide background fluorescence | Glass: Minimal fluorescence Permanox®: Minimal fluorescence Polystyrene: Autofluorescence at <485nm | Glass: • No endogenous fluorescence | Glass: • No endogenous fluorescence |
| Coverslip compatibility | Yes, following gasket removal | Yes | Yes |
| Writing surface | "Sand-blasted" frosted end | Super Frost® white printed end | SuperFrost® blue printed end |
| Growth Surface | Well Size (cm²) | Visible Area (cm²) | Working Volume (mL) |
| 1 Chamber, Glass/Permanox | 9.4 | 9.4 | 2.5-4.5 |
| 2 Chamber, Glass/Permanox | 4.2 | 4.2 | 1.2-2.0 |
| 4 Chamber, Glass/Permanox | 1.8 | 1.8 | 0.5-0.9 |
| 8 Chamber, Glass/Permanox | 0.8 | 0.8 | 0.2-0.4 |
| 16 Chamber, Glass | 0.4 | 0.4 | 0.1-0.2 |
| Flaskette, Glass | 10.0 | 10.0 | 2.5-5.0 |
Lab-Tek® II Chamber Slide™ Specifications | |||
| Growth Surface | Well Size (cm²) | Visible Area (cm²) | Working Volume (mL) |
| 1 Chamber, Glass, CC2 | 10.0 | 8.6 | 4.0-5.0 |
| 2 Chamber, Glass, CC2 | 4.2 | 4.0 | 2.0-2.5 |
| 4 Chamber, Glass, CC2 | 2.4 | 1.7 | 1.0-1.3 |
| 8 Chamber, Glass, CC2 | 1.2 | 0.7 | 0.5-0.6 |
Lab-Tek® Chamber Slide™ System
- Developed for microscopy of in situ cell culture.
- Cells grow on a standard microscope slide.
- Media chamber can be removed when culturing is complete.
- Useful for viral and mycoplasma testing, chromosome studies toxicity tests and immunocytology.
- Permanox® plastic and glass do not interfere with fluorescent labels.
Permanox® is a special molding resin, which meets the growing requirements of most anchorage dependent cells; also excellent chemical resistance allows for cell fixation and the staining of organic solvents for EM and other techniques. Low oxygen permeation makes Permanox® ideal for anoxic cell growth.
Chamber slides combine a media-containing chambered super-structure with a standard soda lime glass slide or Permanox® plastic slide, sealing the two together with a biologically inert silicone gasket. After cells are grown, the chamber and gasket are easily removed. The slide may then be fixed, stained, cover slipped, and filed with the usual convenience. These slides, once fixed and stained, can be embedded using our Slide Duplicating Mold (Cat. 70170).
The Flaskette® chamber (white cap) has a glass slide sealed with a gasket. It is useful with Lowicryl K4M embedding resin, where air inhibits polymerisation of embedding resin. Bou-Gharios, G. et al. J. Microscopy, 150, pt2, 161-163 (1988).
Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.
Note: The chamber slides below are made of glass, but Permanox® is available - ask
Lab-Tek® II Chamber Slide™ products offer optimal surface for excellent attachment and growth of cells, easily distinguished by the blue mark. This design allows for the removal of the media chamber and adhesive from the slide, using a separation device. The blue hydrophobic border is raised slightly and isolates the individual wells, and prevents cross contamination. The slide can be coversliped.
Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.
Documents
Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.
Documents
CC2 shares several similarities with the original Lab-Tek® II Chamber Slide, however, its pronounced difference is that the glass growth surface has been chemically modified. This modification provides an optimal surface for growing fastidious cells such as primary neurons or poorly adherent cell lines. CC2 also are non-auto fluorescent, making them ideal for fluorescent applications such as FISH (fluorescent in situ hybridization). The growth surface remains stable without refrigeration.
Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.
Lab-Tek® and Lab-Tek®II Chambered Coverglass products are intended for high magnification examination of living cells using an inverted microscope. The growth surface of the Lab-Tek Chambered Coverglass products are #1 Borosilicate Coverglass from American origin. The Lab-Tek® II Chambered Coverglass products are #1.5 Borosilicate Coverglass of German origin. The thin glass growth surface allows optimal optical clarity when viewing living cells with high resolution of an inverted microscope. The media chambers are available in 1, 2, 4, and 8 well formats and cannot be removed from the coverglass surface.
The Chambered Coverglass is provided sterile and ready to use
| Product Features | Lab-Tek® | Lab-Tek® II |
| Configurations | 1, 2, 4, 8 well | 1, 2, 4, 8 well |
| Media chamber material | Proprietary cell culture grade polystyrene (PS) | Proprietary cell culture grade polystyrene (PS): non removable |
| Slide Materials | ||
| Cover | Clear polystyrene, no handle, facilitates microscopic viewing | Clear polystyrene, with handle for aseptic manipulations |
| Sealant | Non-toxic medical grade silicone | Non-toxic medical grade silicone |
| Growth Surface | For adherent cell culture. Good growth with fastidious cells, e.g., neurons | For adherent cell culture. Good growth with fastidious cells, e.g., neurons |
| Biological testing | BHK-21, Hep-2 cells | BHK-21, Hep-2 cells |
| Leak tested | Yes | Yes |
| Slide background fluorescence | Glass: Minimal fluorescence | Glass: Minimal fluorescence |
| Coverslip compatibility | Yes, following gasket removal | Yes |
| Writing surface | none | none |
Lab-Tek® Chamber Slides™, Flaskettes® and Chamber Coverglass™ all come in packs of 2 trays, 6 packs/carton. Each sealed, sterile tray has 8 units with either 1, 2, 4, or 8 chambers per unit.
Tissue culture plates are featured on page H5
TCI are small cylinders with a fine, permeable membrane at the base. Cells are grown within the TCI which is immersed in a culture plate with a suitable growth medium. Cells grown on the porous membrane surface exhibit improved attachment, growth, and differentiation over cells grown on plastic surfaces. The medium contacts both the top and the bottom cell layers and, as a result, cells more closely mimic in-vivo structure and function.
Features
- Transparent when wet: This provides optical clarity when viewing cells either microscopically or photographically. Eliminates background interference for better cell differentiation.
- High Porosity: Ensures free diffusion of nutrients to both basolateral (bottom) and apical plasma (top) membranes of attached cells closely resembling an in-vivo environment.
- Solvent Resistant: The cells can be fixed/stained without the need for transferring them with standard solutions.
- Low Background Fluorescence: The membrane will not interfere with fluorescent assays.
- No Matrix Coating Required: Very versatile and it eliminates the coating technique.
TCIs are available in different pore sizes and membrane material:
1. Unique Anaporel ™ Membrane:
This rigid, easy to handle, inorganic membrane offers a combination of excellent transparency when wet and maximum porosity (40-50% surface porosity). The 0.2 micron membrane insures free diffusion of macromolecules with a molecular weight >0.5KD with good transparency characteristics. Anopore membranes can be removed from the polystyrene housing using the Insert removal tool. The membrane is resistant to virtually all solvents. The TCI 10mm is used with 24-well plates; the TCI 25mm is used with 6 well plates; and the 8-well strip insert is used in 96-well plates.
2. Polycarbonate Membrane Inserts:
These polycarbonate (PC) membrane inserts are available in a variety of pore sizes and diameters: 0.4, 3.0, and 8.0 micron in both 10mm and 25mm sizes. PC membranes can be used in many cell culture applications, such as chemotaxis studies where a large pore size is required. The membranes may be removed easily from the insert and are ideal for microtome sectioning.
All TCIs are TC treated, radiation sterilised, and packed individually.