Useful procedures for cryo centrifuge tubes

All vials are made of polypropylene. Some models may contain silicone washers. They can withstand temperatures from +121°C down to -196°C. When freezing, volumes should be 10% less than listed capacity.

When filling, the cryo centrifuge tube should be filled with no more than 90% of the tube's volume (to permit expansion). Upon freezing, ensure that the entire vial is frozen at the same temperature - avoid racks that create partial insulation. The cryo centrifuge tube should be moved periodically during freezing to avoid supercooling and subsequent quick ice formation.
When freezing in liquid nitrogen, ensure that the screw cap is not overtightened. The thread and screw cap of the vial should be completely dry before closing; liquid drops will impair the seal in liquid nitrogen. Vials should be stored in the gas phase above liquid nitrogen. If they are immersed, the vials might develop leaks or even shatter when returned to temperature.
When removing vials from liquid nitrogen containers, always use safety equipment such as gloves, hoods, face shields etc. To thaw, place the vial into a water bath (37-40°C) and keep it in constant motion. As soon as the ice has melted, remove the vial from the water bath.

Legend:
Ø - Diameter

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Useful procedures for cryo centrifuge tubes
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Sterile cryotubes with silicone seal
Sterile cryotubes with silicone seal
CRYOTUBES WITH SILICONE SEAL, STERILE

These cryo tubes have a conical bottom but are free‐standing. Screw cap with silicone seal, external thread, colour varies. ‐ 10B is no longer available.

Cat. No.Volume
(mL)
Volume to top
(mL)
Graduation
(mL)
Tube Height
(mm)
Outer ⌀
(mm)
Inner ⌀
(mm)
Carton Size
YCA17‐50B5.07.00.5 up to 5.05515.51450 x pk/40 = 2000 tubes
YCA17-50B
Cryotubes, PP, sterile, 5.0mL, blue cap with silicone seal
15% DISCOUNT FOR 1
pk/40
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Sterile PP Cryotubes
YT340

CRYOTUBES, PP, SCREW CAP WITH SILICONE SEAL AND CAP INSERT

Features

  • Made from polypropylene for storage as low as ‐196°
  • External thread reduces contamination risk
  • Silicone seal ensures vials are leak‐proof at any temperature
  • Printed graduations
  • White marking area
  • Free standing
  • Cap insert for colour coding
  • Sterile
  • RNase and DNase free
  • Packed in resealable zip‐lock bags
Cat. No.Volume
mL
Graduated to
mL
Graduation
mL
Tube height
mm
Outer ⌀
mm
YT340‐121.21.00.1254212.0
YT340‐202.01.80.14912.0
YT340‐303.53.00.17212.0
YT340‐505.04.50.19112.0
YT340-12B
Cryotubes, PP, sterile, screw cap with O ring, 1.2mL, blue cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-12R
Cryotubes, PP, sterile, screw cap with O ring, 1.2mL, red cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-20B
Cryotubes, PP, sterile, screw cap with O ring, 2.0mL, blue cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-20R
Cryotubes, PP, sterile, screw cap with O ring, 2.0mL, red cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-30B
Cryotubes, PP, sterile, screw cap with O ring, 3.0mL, blue cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-30R
Cryotubes, PP, sterile, screw cap with O ring, 3.0mL, red cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-50B
Cryotubes, PP, sterile, screw cap with O ring, 5.0mL, blue cap insert
15% DISCOUNT FOR 10
pk/100
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YT340-50R
Cryotubes, PP, sterile, screw cap with O ring, 5.0mL, red cap insert
15% DISCOUNT FOR 10
pk/100
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cryolock cryogenic vials
CRYO‐LOK CRYOGENIC VIALS

CRYO‐LOK vials are ideal for storage or transport of material of extremely low temperatures. The vials have external threads, providing a smooth and uniform inner surface. This eliminates the problem of air born contamination and/or sample loss so common when vials with internal threads are used. The screw top incorporates a molded‐in sealing ring, which assures a tight leak‐proof seal.


  • Graduated in 0.5 mL increments
  • Conical bottom with slotted skirt
  • For use in vapour phase of liquid nitrogen
  • Other coloured caps are also available ‐ ask.
Y72650-B
Cryo-Lok vials, 2mL non-sterile, blue
10% DISCOUNT FOR 1
pk/1000
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Polypropylene caps for cryovials
YT312
CAPINSERT™

polypropylene

  • Colour identification inserts for Cryovial® tubes
  • Colour inserts fit precisely into the Cryovial® cap.
  • Colour coding for date, content, process etc. is possible by this means.
  • Thermal characteristics of cap inserts are identical to Cryovial®.
  • Low temperatures to ‐196°C
YT312-B
Capinsert™ for cryovials, blue
10% DISCOUNT FOR 5
pk/100
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YT312-R
Capinsert™ for cryovials, red
10% DISCOUNT FOR 5
pk/100
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YT312-W
Capinsert™ for cryovials, white
10% DISCOUNT FOR 5
pk/100
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YT312-Y
Capinsert™ for cryovials, yellow
10% DISCOUNT FOR 5
pk/100
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Cryotube box
YC20 - Cryotube box
CRYOTUBE BOX

Polycarbonate, -100°C

This cryo box is made of white PC. The lid matches the base in one rotation only, and an index identifying tubes is provided. The holes take our 1.0 and 1.8mL cryotubes, also small centrifuge tubes on page L9
See also microtube storage boxes on page L12.
Hole size: Ø13mm, H50mm.

YC20
Cryotube box, PC, for 100 x 1mL - 2mL tubes
10% DISCOUNT FOR 10
ea
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Cryotube box
Cryotube box
CRYOTUBE BOX, CLEAR LID

Polycarbonate, -100°C

Moulded polycarbonate boxes with positive alignment of lift-off lid versus box. Lids may be used to index specimen tubes. PC is strong and remains non-brittle in very low temperature freezers. Suitable for temperatures from -190°C to +121°C. The boxes are vented and stackable. The transparent cover allows view of contents and is numbered. The rack insert has numbered holes and may be blue, red, orange or yellow.

YC23-050
Cryotube box, PC, for 50 x 1mL - 2mL tubes, clear lid
ea
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YC23-081
Cryotube box, PC, for 81 x 1mL - 2mL tubes, clear lid
ea
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YC23-100
Cryotube box, PC, for 100 x 1mL - 2mL tubes, clear lid
ea
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YC24-081
Cryotube box, PC, for 81 x 5mL tubes, clear lid
ea
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Cardboard cryotube storage boxes
YC21-016
Cardboard cryotube storage boxes
YC21-064
CRYOTUBE BOXES

CARDBOARD

  • Designed for storage of samples at ultra low temperatures
  • Made of treated white cardboard and covered with coated, moisture resistant, white paper
  • Pre‐assembled compartments offer ideal, indexed space for many tube sizes
  • No brittleness temperature effect: superior at lower temperatures.
Cat. No.Dimensions with lid, mmHole size, mmNumber of holesAlphanumeric labelling
YC21‐016150 x 150 x 12434 x 3416yes
YC21‐049136 x 136 x 11318 x 1847yes
YC21‐064134 x 134 x 4815 x 1564no
YC21‐081134 x 134 x 4813 x 1381no
YC21‐100134 x 134 x 4811 x 11100no
YC21-016
Cryotube boxes, cardboard, for 16 x 50mL tubes
10% DISCOUNT FOR 6
pk/4
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YC21-049
Cryotube boxes, cardboard, for 49 x 15mL tubes
10% DISCOUNT FOR 6
pk/4
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YC21-064
Cryotube boxes, cardboard, for 64 x 1.8 - 2mL microtubes
10% DISCOUNT FOR 6
pk/4
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YC21-081
Cryotube boxes, cardboard, for 81 x 1.8 -2mL microtubes
10% DISCOUNT FOR 6
pk/4
SHOP
YC21-100
Cryotube boxes, cardboard, for 100 x 1mL microtubes
10% DISCOUNT FOR 6
pk/4
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Cardboard cryotube storage boxes
YC22

CRYO BOXES

Cardboard

  • Designed for storage of samples at ultra low temperatures
  • Made of treated white cardboard and covered with coated, moisture resistant, white paper
  • Pre‐assembled compartments offer ideal, indexed space for many tube sizes
  • No brittleness temperature effect: superior at lower temperatures.
Cat. No.Dimensions with lid, mmHole size, mmNumber of holes
YC22‐064134 x 134 x 7715 x 1564
YC22‐081134 x 134 x 7713 x 1381
YC22‐100134 x 134 x 7711 x 11100
YC22-064
Cryotube boxes, cardboard, for 64 x 75mm tubes
10% DISCOUNT FOR 6
pk/4
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YC22-081
Cryotube boxes, cardboard, for 81 x 75mm tubes
pk/4
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YC22-100
Cryotube boxes, cardboard, for 100 x 75mm tubes
pk/4
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Cryo grid box with lid
Cryo grid box with lid
Handling rod
Handling rod

CRYO GRID STORAGE BOX WITH LID, ROUND

The cryo grid box is used for transferring, storing and manipulating vitrified cryo TEM specimens (i.e. on Quantifoil TEM grids) made with cryo-devices like the FEI Vitrobot, Gatan CP3, Leica EM 6P and other cryo vitrification systems. The round box is the most widely used and is suitable for the Gatan 626 and CT3500 cryo transfer systems and the FEI Vitrobot and Gatan CP3. The box has a UNC 5/40 thread in the center. The clear lid is held in place with a stainless steel screw.

The handling rod screws into the threaded centre of the round cryo grid box for pick-up and storage applications. Can be used to store the cryo grid box in liquid nitrogen.

YL070
Cryo grid storage box with lid, round
10% DISCOUNT FOR 10
ea
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YL070-R
Handling rod for cryo grid box
ea
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Click here for Apiezon N and other low-temperature, conducting or sticky greases.

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Link to Apiezon greases
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CANE FOR CRYOVIAL® TUBES

aluminium 5-place

For storing up to five 1.2mL or 2mL Simport Cryovial® tubes in liquid nitrogen freezers such as Dewar flasks.
YT313

YT313 can be used with YT240 series.
YT313 fits 6 YT240‐12 or 4/5 YT240‐20 or 3 YT240‐30 or 3 YT240‐50

YT313
Cane for Cryovial tubes
pk/10
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Disposable plastic Cryomoulds®
Y565, Y566, Y557
TISSUE-TEK® CRYOMOLD® SAKURA FINETEK

These disposable plastic Cryomoulds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® moulds to freezing wells on the Cryobar® unit.


Cat. No.
Type
Length (mm)
Width (mm)
Depth (mm)
Pack size
Y557
Standard
25
20
5
100 moulds
Y565
Biopsy
10
10
5
100 moulds
Y566
Intermediate
15
15
5
100 moulds

See also these items:

20% discount for a carton of 12 packs

Y557
Tissue-Tek® Cryomolds®, standard, 25 x 20 x 5mm
10% DISCOUNT FOR 4
pk/100
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Y565
Tissue-Tek® Cryomolds®, biopsy, 10 x 10 x 5mm
10% DISCOUNT FOR 4
pk/100
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Y566
Tissue-Tek® Cryomolds®, intermediate, 15 x 15 x 5mm
10% DISCOUNT FOR 4
pk/100
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Cryomoulds
ACCESSORIES FOR TISSUE-TEK® CRYO3® MICROTOME/CRYOSTAT CRYO 2000™

CRYOMOLD® SPECIMEN HOLDERS

Plastic Cryomoulds, round shapes with a tab for easy handling. Available in standard and biopsy configurations.

The standard mould measures 22mm (D) x 5mm deep.

The biopsy mould measures 15mm (D) x 5mm deep.

Y631
Cryo3 Cryomolds, standard, 22mm
10% DISCOUNT FOR 12
pk/100
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Y632
Cryo3 Cryomolds, biopsy, 15mm
15% DISCOUNT FOR 12
pk/100
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